Antares-SEPLuc IRESv24

LOCUS      Antares-SEPLuc IRESv24               9157 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  precursor of the bioluminescent reporter pHluc, for detecting 
            extracellular pH changes in tumor acidosis; utilizes weaker mutant 
            of IRES.
ACCESSION   .
VERSION     .
KEYWORDS    Antares-SEPLuc IRESv24
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL
  TITLE     pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of 
            Tumor Acidosis.
  JOURNAL   Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 
            10.3389/fbioe.2020.00412. eCollection 2020.
  PUBMED    32457886
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(57..76)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        248..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..831
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     782..802
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     876..895
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        876..894
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1655..2161
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="FTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIV
                     LSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTP
                     NMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRL
                     CERILA"
     CDS             2861..2914
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2921..3520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2921..2940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3417..3437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3522..4095
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3689..3706)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3916..3935
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5556..5573)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5562..5786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5832..6260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5919..5938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6129..6150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6269..6289)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6274..6603
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6454..6589
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6516..6535
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    6843..6964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6880..6899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6934..6953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7013..7029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7013..7029)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7026..7048)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7037..7053
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7061..7091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7106..7127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7244..7261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7415..8003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7495..7514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8174..9034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8797..8816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9035..9139)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
       61 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
      121 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
      181 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      241 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      301 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      361 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      421 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      481 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      541 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      601 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      661 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      721 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      781 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      841 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      901 caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      961 ggtggaattc atggtgagca agggcgagga gctgatcaag gagaacatga gaagcaagct
     1021 gtacctggaa ggcagcgtga acggccacca gttcaagtgc acccacgaag gggagggcaa
     1081 gccctacgag ggcaagcaga ccaacaggat caaggtggtg gagggaggcc ccctgccgtt
     1141 cgcattcgac atcctggcca cccactttat gtacgggagc aaggtgttca tcaagtaccc
     1201 cgccgacctc cccgattatt ttaagcagtc cttccctgag ggcttcacat gggagagagt
     1261 catggtgttc gaagacgggg gcgtgctgac cgccacccag gacaccagcc tccaggacgg
     1321 cgagctcatc tacaacgtca aggtcagagg ggtgaacttc ccagccaacg gccccgtgat
     1381 gcagaagaaa acactgggct gggagcccag caccgagacc atgtaccccg ctgacggcgg
     1441 cctggaaggc agatgcgaca aggccctgaa gctcgtgggc gggggccacc tgcacgtcaa
     1501 cttcaagacc acatacaagt ccaagaaacc cgtgaagatg cccggcgtcc actacgtgga
     1561 ccgcagactg gaaagaatca aggaggccga caacgagacc tacgtcgagc agtacgagca
     1621 cgctgtggcc agatactcca acctgggcgg aggcttcaca ctcgaagatt tcgttgggga
     1681 ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag
     1741 tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga
     1801 aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca
     1861 aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa
     1921 ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta
     1981 tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg
     2041 gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct
     2101 gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc
     2161 gaggcacgag ctgatcaagg agaacatgag aagcaagctg tacctggaag gcagcgtgaa
     2221 cggccaccag ttcaagtgca cccacgaagg ggagggcaag ccctacgagg gcaagcagac
     2281 caacaggatc aaggtggtgg agggaggccc cctgccgttc gcattcgaca tcctggccac
     2341 ccactttatg tacgggagca aggtgttcat caagtacccc gccgacctcc ccgattattt
     2401 taagcagtcc ttccctgagg gcttcacatg ggagagagtc atggtgttcg aagacggggg
     2461 cgtgctgacc gccacccagg acaccagcct ccaggacggc gagctcatct acaacgtcaa
     2521 ggtcagaggg gtgaacttcc cagccaacgg ccccgtgatg cagaagaaaa cactgggctg
     2581 ggagcccagc accgagacca tgtaccccgc tgacggcggc ctggaaggca gatgcgacaa
     2641 ggccctgaag ctcgtgggcg ggggccacct gcacgtcaac ttcaagacca catacaagtc
     2701 caagaaaccc gtgaagatgc ccggcgtcca ctacgtggac cgcagactgg aaagaatcaa
     2761 ggaggccgac aacgagacct acgtcgagca gtacgagcac gctgtggcca gatactccaa
     2821 cctgggcgga ggcatggacg agctgtacaa ggcggccgct gagggcagag gaagtcttct
     2881 aacatgcggt gacgtggagg agaatcccgg cccttccggg atgaccgagt acaagcccac
     2941 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     3001 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     3061 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3121 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3181 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3241 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3301 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3361 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3421 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3481 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga acccctctcc ctcccccccc
     3541 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     3601 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     3661 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     3721 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     3781 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     3841 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     3901 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     3961 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     4021 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     4081 aaaacacgat gataatgtga tggttgagat gttgccaacc gtagctgtgc tcgttttggc
     4141 tgtcagcgta gtcgctaaag ataatacaac acttcaggag ttcgcaacat ctaagggaga
     4201 agagttgttt actggcgtag tacccattct tgtggaactt gacggcgatg ttaatggtca
     4261 taagttctct gtgtctggtg agggcgaggg ggatgctaca tatggtaaat tgaccctcaa
     4321 atttatttgt acaaccggga agctccctgt accctggcct actctcgtga ccaccctcac
     4381 ctacggggta caatgttttt ccagataccc cgatcacatg aagcgccacg atttcttcaa
     4441 aagtgccatg cccgagggtt acgtccagga acgcacaata tttttcaagg atgatggaaa
     4501 ttacaaaacc cgcgccgagg ttaagttcga gggggatacc ctcgttaacc ggattgaact
     4561 gaagggtatc gactttaagg aggacggtaa tatattgggg cataaattgg aatacaacta
     4621 taacgaccac caggtgtata tcatggccga taaacagaag aacggcatca aagcaaactt
     4681 taaaattagg cataacatag aggatggtgg cgttcagctt gctgatcact atcaacagaa
     4741 tacccctata ggtgatggac ccgtgctcct cccagataac cattacttgt ttacaacttc
     4801 tacactctct aaggacccca acgaaaagag agaccacatg gtgctcttgg agttcgtaac
     4861 tgccgccgga attacacatg gtatggacga gctctacaag gatatttccg ggggggtctt
     4921 tacacttgaa gatttcgtcg gggattggag acaaactgcc ggttataatc ttgatcaggt
     4981 gcttgagcaa gggggggtga gctccctttt tcaaaacctg ggtgtatctg tcacccctat
     5041 ccaaaggatt gtgcttagcg gtgaaaatgg tctcaaaatc gacatccacg tgatcatccc
     5101 atacgagggt cttagtggag atcagatggg gcagatagag aagattttca aagttgttta
     5161 ccctgtcgac gaccaccatt tcaaagtaat tctgcactac ggaaccctcg tcatagacgg
     5221 ggtcacaccc aacatgatag attactttgg gcgaccctat gaagggatag cagtattcga
     5281 cggcaaaaag ataaccgtga caggcaccct gtggaatgga aacaaaatta tagatgagcg
     5341 cctgatcaac cctgacggtt ctttgttgtt cagggttaca atcaatggtg tcacaggctg
     5401 gaggctctgt gagcgcatcc tggctcagtt ggagaatggg gggatctccc tcttggtgca
     5461 aaatacatca tggatgttgc ttcttcttct tagcttgagc ctcttgcagg cattggattt
     5521 catcagtctc taacccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5581 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5641 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5701 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5761 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5821 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5881 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5941 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6001 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6061 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6121 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6181 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6241 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6301 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6361 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6421 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6481 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6541 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6601 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     6661 tcgtttcgcg cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6721 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6781 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6841 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6961 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9121 aacaaatagg ggttccgcgc acatttcccc gaaaagt
//